Dna may be denatured if diluted in dh 2 o.
Bio rad 20 bp ladder.
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Standard ez load and amplisize rulers.
The size markers used were a 100 bp ladder ez load 100 pb pcr molecular ruler biorad marnes la coquette france or 20 bp ladder ez load 20 pb molecular ruler biorad marnes la coquette france according to the tandem repeat unit length.
Monitor electrophoresis and blotting experiments estimate molecular weights.
500 μl 0 05 μg μl ready to load dna standard 100 1 000 bp 10 bands includes 1 ml 5x nucleic acid sample buffer 100 applications.
We recommend loading 0 5 1 0 µg of 50 bp dna ladder diluted in sample buffer.
Bio rad provides three types of molecular rulers.
200 μl 0 2 μg μl dna standard 500 8 000 bp 16 bands in 500 bp increments 100 applications.
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If samples need to be diluted use te or other buffer of minimal ionic strength.
Molecular mass rulers bio rad precision molecular mass rulers dna ladders allow accurate dna quantitation in gels making them ideal for densitometry or image analysis.
Bio rad provides three types of molecular rulers.
Gel images were managed using the bionumerics software package version 4 0 applied maths sint.
Or 0 5 2 5 µl for silver staining up to 1 000 applications.
Use 1 0 5 0 µl for full length gels 16 20 cm to yield 100 or up to 500.
Five μl of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.
For long term storage store at 20 c.
50 bp dna ladder is stable for at least 3 months at 4 c.
1x gel loading dye purple no sds.
Bio rad molecular rulers are dna standards with precisely defined size intervals between bands dna ladders for simplified estimation of the length of nucleic acids separated on agarose gels.
Standard ez load and amplisize rulers.
